Part:BBa_K3425040:Design

β-subunit of Qβ replicase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1314
  Illegal XhoI site found at 822
  Illegal XhoI site found at 988
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Silent mutations were engineered in order to remove the restriction sites: PstI, EcoRI and two sites of BpiI. This ensured compatibility with Type IIS iGEM standard assembly, BioBrick assembly and other Type IIS cloning techniques utilizing BpiI restriction enzyme. You can also read more about how this part was cloned in the Type IIS guidebook of Uppsala 2020 team.

Source

Synthesized according to
[1] Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078

References

[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078